Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase

BACKGROUND: Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. RESULTS: In a yeast t-degron (ubiquitin-argDHFR(ts))- phosphoglycerate mutase (GPM1) fusion strain, increasing period...

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Detalles Bibliográficos
Autores principales: Heidrich, Katja, Fraenkel, Dan G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC117797/
https://www.ncbi.nlm.nih.gov/pubmed/12149134
http://dx.doi.org/10.1186/1471-2156-3-13

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC117797/
https://www.ncbi.nlm.nih.gov/pubmed/12149134
http://dx.doi.org/10.1186/1471-2156-3-13

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