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Setting of graded levels of a protein in yeast by a t-degron technique as applied to phosphoglycerate mutase
BACKGROUND: Setting of graded levels of a protein for in vivo studies by controlled gene expression has inconveniences, and we here explore the use of the t-degron technique instead. RESULTS: In a yeast t-degron (ubiquitin-argDHFR(ts))- phosphoglycerate mutase (GPM1) fusion strain, increasing period...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2002
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC117797/ https://www.ncbi.nlm.nih.gov/pubmed/12149134 http://dx.doi.org/10.1186/1471-2156-3-13 |