Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5′-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi

To search for restriction endonucleases, we used a novel plant-based cell-free translation procedure that bypasses the toxicity of these enzymes. To identify candidate genes, the related genomes of the hyperthermophilic archaea Pyrococcus abyssi and Pyrococcus horikoshii were compared. In line with...

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Detalles Bibliográficos
Autores principales: Ishikawa, Ken, Watanabe, Miki, Kuroita, Toshihiro, Uchiyama, Ikuo, Bujnicki, Janusz M., Kawakami, Bunsei, Tanokura, Masaru, Kobayashi, Ichizo
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178009/
https://www.ncbi.nlm.nih.gov/pubmed/16040595
http://dx.doi.org/10.1093/nar/gni113

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1178009/
https://www.ncbi.nlm.nih.gov/pubmed/16040595
http://dx.doi.org/10.1093/nar/gni113

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