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Development of ERK Activity Sensor, an in vitro, FRET-based sensor of Extracellular Regulated Kinase activity
BACKGROUND: Study of ERK activation has thus far relied on biochemical assays that are limited to the use of phospho-specific antibodies and radioactivity in vitro, and analysis of whole cell populations in vivo. As with many systems, fluorescence resonance energy transfer (FRET) can be utilized to...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1180429/ https://www.ncbi.nlm.nih.gov/pubmed/15998468 http://dx.doi.org/10.1186/1472-6769-5-1 |