Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products

In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with grea...

Descripción completa

Detalles Bibliográficos
Autores principales: Shigemori, Yasushi, Mikawa, Tsutomu, Shibata, Takehiko, Oishi, Michio
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183492/
https://www.ncbi.nlm.nih.gov/pubmed/16087733
http://dx.doi.org/10.1093/nar/gni111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183492/
https://www.ncbi.nlm.nih.gov/pubmed/16087733
http://dx.doi.org/10.1093/nar/gni111

Ejemplares similares