Determination of the relative amounts of Gag and Pol proteins in foamy virus particles

We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68(Gag )moiety dominates in infectious FVs. Furthermore, approximate...

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Detalles Bibliográficos
Autores principales: Cartellieri, Marc, Rudolph, Wolfram, Herchenröder, Ottmar, Lindemann, Dirk, Rethwilm, Axel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1185564/
https://www.ncbi.nlm.nih.gov/pubmed/16004609
http://dx.doi.org/10.1186/1742-4690-2-44
Descripción
Sumario:We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68(Gag )moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71(Gag )plus p68(Gag):p85(RT)),12:1 (p68(Gag):p85(RT)), and 10:1 (pr71(Gag )plus p68(Gag):p40(IN)). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.

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