Determination of the relative amounts of Gag and Pol proteins in foamy virus particles

We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68(Gag )moiety dominates in infectious FVs. Furthermore, approximate...

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Autores principales: Cartellieri, Marc, Rudolph, Wolfram, Herchenröder, Ottmar, Lindemann, Dirk, Rethwilm, Axel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1185564/
https://www.ncbi.nlm.nih.gov/pubmed/16004609
http://dx.doi.org/10.1186/1742-4690-2-44
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author Cartellieri, Marc
Rudolph, Wolfram
Herchenröder, Ottmar
Lindemann, Dirk
Rethwilm, Axel
author_facet Cartellieri, Marc
Rudolph, Wolfram
Herchenröder, Ottmar
Lindemann, Dirk
Rethwilm, Axel
author_sort Cartellieri, Marc
collection PubMed
description We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68(Gag )moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71(Gag )plus p68(Gag):p85(RT)),12:1 (p68(Gag):p85(RT)), and 10:1 (pr71(Gag )plus p68(Gag):p40(IN)). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.
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spelling pubmed-11855642005-08-13 Determination of the relative amounts of Gag and Pol proteins in foamy virus particles Cartellieri, Marc Rudolph, Wolfram Herchenröder, Ottmar Lindemann, Dirk Rethwilm, Axel Retrovirology Short Report We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68(Gag )moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71(Gag )plus p68(Gag):p85(RT)),12:1 (p68(Gag):p85(RT)), and 10:1 (pr71(Gag )plus p68(Gag):p40(IN)). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy. BioMed Central 2005-07-08 /pmc/articles/PMC1185564/ /pubmed/16004609 http://dx.doi.org/10.1186/1742-4690-2-44 Text en Copyright © 2005 Cartellieri et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Cartellieri, Marc
Rudolph, Wolfram
Herchenröder, Ottmar
Lindemann, Dirk
Rethwilm, Axel
Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title_full Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title_fullStr Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title_full_unstemmed Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title_short Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
title_sort determination of the relative amounts of gag and pol proteins in foamy virus particles
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1185564/
https://www.ncbi.nlm.nih.gov/pubmed/16004609
http://dx.doi.org/10.1186/1742-4690-2-44
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