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Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs

Direct site-specific biotinylation of RNA molecules was achieved by specific transcription mediated by unnatural base pairs. Unnatural base pairs between 2-amino-6-(2-thienyl)purine (denoted by s) and 2-oxo(1H)pyridine (denoted by y), or 2-amino-6-(2-thiazolyl)purine (denoted as v) and y specificall...

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Detalles Bibliográficos
Autores principales: Moriyama, Kei, Kimoto, Michiko, Mitsui, Tsuneo, Yokoyama, Shigeyuki, Hirao, Ichiro
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1188086/
https://www.ncbi.nlm.nih.gov/pubmed/16113238
http://dx.doi.org/10.1093/nar/gni128