Cargando…

An encoded N-terminal extension results in low levels of heterologous protein production in Escherichia coli

BACKGROUND: The tdk gene (encoding deoxythymidine kinase) of the gamma-proteobacterium Xenorhabdus nematophila has two potential translation start sites. The promoter-distal start site was predicted to be functional based on amino acid sequence alignment with closely related Tdk proteins. However, t...

Descripción completa

Detalles Bibliográficos
Autores principales: Orchard, Samantha S, Goodrich-Blair, Heidi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190211/
https://www.ncbi.nlm.nih.gov/pubmed/16042769
http://dx.doi.org/10.1186/1475-2859-4-22