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An encoded N-terminal extension results in low levels of heterologous protein production in Escherichia coli
BACKGROUND: The tdk gene (encoding deoxythymidine kinase) of the gamma-proteobacterium Xenorhabdus nematophila has two potential translation start sites. The promoter-distal start site was predicted to be functional based on amino acid sequence alignment with closely related Tdk proteins. However, t...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1190211/ https://www.ncbi.nlm.nih.gov/pubmed/16042769 http://dx.doi.org/10.1186/1475-2859-4-22 |