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Subunit assembly modulates the activities of the Type III restriction–modification enzyme PstII in vitro
We demonstrate that, like other Type III restriction endonuclease, PstII does not turnover such that a DNA substrate is only fully cleaved at a Res(2)Mod(2)-to-site ratio of ∼1:1. However, unlike other Type III enzymes, the cleavage rate profiles varied with protein concentration: using 5 nM DNA and...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1192831/ https://www.ncbi.nlm.nih.gov/pubmed/16120968 http://dx.doi.org/10.1093/nar/gki788 |