Trapping DNA–protein binding reactions with neutral osmolytes for the analysis by gel mobility shift and self-cleavage assays

We take advantage of our previous observation that neutral osmolytes can strongly slow down the rate of DNA–protein complex dissociation to develop a method that uses osmotic stress to ‘freeze’ mixtures of DNA–protein complexes and prevent further reaction enabling analysis of the products. We apply...

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Detalles Bibliográficos
Autores principales: Sidorova, Nina Y., Muradymov, Shakir, Rau, Donald C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214545/
https://www.ncbi.nlm.nih.gov/pubmed/16155185
http://dx.doi.org/10.1093/nar/gki808

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1214545/
https://www.ncbi.nlm.nih.gov/pubmed/16155185
http://dx.doi.org/10.1093/nar/gki808

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