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Bacillus cereus DNA topoisomerase I and IIIα: purification, characterization and complementation of Escherichia coli TopoIII activity

The Bacillus cereus genome possesses three type IA topoisomerase genes. These genes, encoding DNA topoisomerase I and IIIα (bcTopo I, bcTopo IIIα), have been cloned into T7 RNA polymerase-regulated plasmid expression vectors and the enzymes have been overexpressed, purified and characterized. The pr...

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Detalles Bibliográficos
Autores principales: Li, Zhiyu, Hiasa, Hiroshi, DiGate, Russell
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236973/
https://www.ncbi.nlm.nih.gov/pubmed/16192570
http://dx.doi.org/10.1093/nar/gki846