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Real-time expression profiling of microRNA precursors in human cancer cell lines

Our previous study described a real-time PCR method to quantify microRNA (miRNA) precursors using SYBR green detection [T. D. Schmittgen, J. Jiang, Q. Liu and L. Yang (2004) Nucleic Acids Res., 32, e43]. The present study adapted the assay to a 384-well format and expanded it to include primers to 2...

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Detalles Bibliográficos
Autores principales: Jiang, Jinmai, Lee, Eun Joo, Gusev, Yuriy, Schmittgen, Thomas D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1236977/
https://www.ncbi.nlm.nih.gov/pubmed/16192569
http://dx.doi.org/10.1093/nar/gki863