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Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule

V(D)J recombination at the immunoglobulin heavy chain (IgH) locus follows the 12/23 rule to ensure the correct assembly of the variable region gene segments. Here, we report characterization of an in vivo model that allowed us to study recombination violating the 12/23 rule, namely a mouse strain la...

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Autores principales: Koralov, Sergei B., Novobrantseva, Tatiana I., Hochedlinger, Konrad, Jaenisch, Rudolf, Rajewsky, Klaus
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1237122/
https://www.ncbi.nlm.nih.gov/pubmed/15699070
http://dx.doi.org/10.1084/jem.20041577
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author Koralov, Sergei B.
Novobrantseva, Tatiana I.
Hochedlinger, Konrad
Jaenisch, Rudolf
Rajewsky, Klaus
author_facet Koralov, Sergei B.
Novobrantseva, Tatiana I.
Hochedlinger, Konrad
Jaenisch, Rudolf
Rajewsky, Klaus
author_sort Koralov, Sergei B.
collection PubMed
description V(D)J recombination at the immunoglobulin heavy chain (IgH) locus follows the 12/23 rule to ensure the correct assembly of the variable region gene segments. Here, we report characterization of an in vivo model that allowed us to study recombination violating the 12/23 rule, namely a mouse strain lacking canonical D elements in its IgH locus. We demonstrate that V(H) to J(H) joining can support the generation of all B cell subsets. However, the process is inefficient in that B cells and antibodies derived from the D(H)-less allele are not detectable if the latter is combined with a wild-type IgH allele. There is no preferential usage of any particular V(H) gene family or J(H) element in V(H)J(H) junctions, indicating that 23/23-guided recombination is possible, but is a low frequency event at the IgH locus in vivo.
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spelling pubmed-12371222008-03-11 Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule Koralov, Sergei B. Novobrantseva, Tatiana I. Hochedlinger, Konrad Jaenisch, Rudolf Rajewsky, Klaus J Exp Med Brief Definitive Report V(D)J recombination at the immunoglobulin heavy chain (IgH) locus follows the 12/23 rule to ensure the correct assembly of the variable region gene segments. Here, we report characterization of an in vivo model that allowed us to study recombination violating the 12/23 rule, namely a mouse strain lacking canonical D elements in its IgH locus. We demonstrate that V(H) to J(H) joining can support the generation of all B cell subsets. However, the process is inefficient in that B cells and antibodies derived from the D(H)-less allele are not detectable if the latter is combined with a wild-type IgH allele. There is no preferential usage of any particular V(H) gene family or J(H) element in V(H)J(H) junctions, indicating that 23/23-guided recombination is possible, but is a low frequency event at the IgH locus in vivo. The Rockefeller University Press 2005-02-07 /pmc/articles/PMC1237122/ /pubmed/15699070 http://dx.doi.org/10.1084/jem.20041577 Text en Copyright © 2005, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Report
Koralov, Sergei B.
Novobrantseva, Tatiana I.
Hochedlinger, Konrad
Jaenisch, Rudolf
Rajewsky, Klaus
Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title_full Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title_fullStr Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title_full_unstemmed Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title_short Direct in vivo V(H) to J(H) rearrangement violating the 12/23 rule
title_sort direct in vivo v(h) to j(h) rearrangement violating the 12/23 rule
topic Brief Definitive Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1237122/
https://www.ncbi.nlm.nih.gov/pubmed/15699070
http://dx.doi.org/10.1084/jem.20041577
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