Cargando…

Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.

Exposure to di-(2-ethylhexyl) phthalate (DEHP) is prevalent based on the measurement of its hydrolytic metabolite mono-(2-ethylhexyl) phthalate (MEHP) in the urine of 78% of the general U.S. population studied in the 1999-2000 National Health and Nutrition Examination Survey (NHANES). However, despi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kato, Kayoko, Silva, Manori J, Reidy, John A, Hurtz, Donald, Malek, Nicole A, Needham, Larry L, Nakazawa, Hiroyuki, Barr, Dana B, Calafat, Antonia M
Formato: Texto
Lenguaje:English
Publicado: 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1241862/
https://www.ncbi.nlm.nih.gov/pubmed/14998748
_version_ 1782125523613253632
author Kato, Kayoko
Silva, Manori J
Reidy, John A
Hurtz, Donald
Malek, Nicole A
Needham, Larry L
Nakazawa, Hiroyuki
Barr, Dana B
Calafat, Antonia M
author_facet Kato, Kayoko
Silva, Manori J
Reidy, John A
Hurtz, Donald
Malek, Nicole A
Needham, Larry L
Nakazawa, Hiroyuki
Barr, Dana B
Calafat, Antonia M
author_sort Kato, Kayoko
collection PubMed
description Exposure to di-(2-ethylhexyl) phthalate (DEHP) is prevalent based on the measurement of its hydrolytic metabolite mono-(2-ethylhexyl) phthalate (MEHP) in the urine of 78% of the general U.S. population studied in the 1999-2000 National Health and Nutrition Examination Survey (NHANES). However, despite the high level of production and use of DEHP, the urinary MEHP levels in the NHANES samples were lower than the monoester metabolites of phthalates less commonly used than DEHP, suggesting metabolic differences between phthalates. We measured MEHP and two oxidative DEHP metabolites, mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) to verify whether these other metabolites account for a greater proportion of DEHP metabolic products in 127 paired human urine and serum samples. We found that the urinary levels of MEHHP and MEOHP were 10-fold higher than levels of MEHP; concentrations of urinary MEOHP and MEHHP were strongly correlated (r = 0.928). We also found that the serum levels of MEOHP and MEHHP were comparatively lower than those in urine. Furthermore, the glucuronide-bound conjugates of the oxidative metabolites were the predominant form in both urine and serum. MEOHP and MEHHP cannot be formed by serum enzymes from the hydrolysis of any contamination from DEHP potentially introduced during blood collection and storage. Therefore, concentrations of MEHHP and MEOHP in serum may be a more selective measure of DEHP exposure than is MEHP. However, additional data on the absorption, distribution, metabolism, and elimination of these oxidative metabolites are needed to completely understand the extent of DEHP exposure from the serum concentrations of oxidative DEHP metabolites.
format Text
id pubmed-1241862
institution National Center for Biotechnology Information
language English
publishDate 2004
record_format MEDLINE/PubMed
spelling pubmed-12418622005-11-08 Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate. Kato, Kayoko Silva, Manori J Reidy, John A Hurtz, Donald Malek, Nicole A Needham, Larry L Nakazawa, Hiroyuki Barr, Dana B Calafat, Antonia M Environ Health Perspect Research Article Exposure to di-(2-ethylhexyl) phthalate (DEHP) is prevalent based on the measurement of its hydrolytic metabolite mono-(2-ethylhexyl) phthalate (MEHP) in the urine of 78% of the general U.S. population studied in the 1999-2000 National Health and Nutrition Examination Survey (NHANES). However, despite the high level of production and use of DEHP, the urinary MEHP levels in the NHANES samples were lower than the monoester metabolites of phthalates less commonly used than DEHP, suggesting metabolic differences between phthalates. We measured MEHP and two oxidative DEHP metabolites, mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) and mono (2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) to verify whether these other metabolites account for a greater proportion of DEHP metabolic products in 127 paired human urine and serum samples. We found that the urinary levels of MEHHP and MEOHP were 10-fold higher than levels of MEHP; concentrations of urinary MEOHP and MEHHP were strongly correlated (r = 0.928). We also found that the serum levels of MEOHP and MEHHP were comparatively lower than those in urine. Furthermore, the glucuronide-bound conjugates of the oxidative metabolites were the predominant form in both urine and serum. MEOHP and MEHHP cannot be formed by serum enzymes from the hydrolysis of any contamination from DEHP potentially introduced during blood collection and storage. Therefore, concentrations of MEHHP and MEOHP in serum may be a more selective measure of DEHP exposure than is MEHP. However, additional data on the absorption, distribution, metabolism, and elimination of these oxidative metabolites are needed to completely understand the extent of DEHP exposure from the serum concentrations of oxidative DEHP metabolites. 2004-03 /pmc/articles/PMC1241862/ /pubmed/14998748 Text en
spellingShingle Research Article
Kato, Kayoko
Silva, Manori J
Reidy, John A
Hurtz, Donald
Malek, Nicole A
Needham, Larry L
Nakazawa, Hiroyuki
Barr, Dana B
Calafat, Antonia M
Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title_full Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title_fullStr Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title_full_unstemmed Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title_short Mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
title_sort mono(2-ethyl-5-hydroxyhexyl) phthalate and mono-(2-ethyl-5-oxohexyl) phthalate as biomarkers for human exposure assessment to di-(2-ethylhexyl) phthalate.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1241862/
https://www.ncbi.nlm.nih.gov/pubmed/14998748
work_keys_str_mv AT katokayoko mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT silvamanorij mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT reidyjohna mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT hurtzdonald mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT maleknicolea mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT needhamlarryl mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT nakazawahiroyuki mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT barrdanab mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate
AT calafatantoniam mono2ethyl5hydroxyhexylphthalateandmono2ethyl5oxohexylphthalateasbiomarkersforhumanexposureassessmenttodi2ethylhexylphthalate