PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets

We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as...

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Detalles Bibliográficos
Autores principales: Kojima, Takaaki, Takei, Yoshiaki, Ohtsuka, Miharu, Kawarasaki, Yasuaki, Yamane, Tsuneo, Nakano, Hideo
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1251669/
https://www.ncbi.nlm.nih.gov/pubmed/16214800
http://dx.doi.org/10.1093/nar/gni143

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1251669/
https://www.ncbi.nlm.nih.gov/pubmed/16214800
http://dx.doi.org/10.1093/nar/gni143

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