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Position of the fluorescent label is a crucial factor determining signal intensity in microarray hybridizations

A key issue in applications of short oligonucleotide-based microarrays is how to design specific probes with high sensitivity. Some details of the factors affecting microarray hybridization remain unclear, hampering a reliable quantification of target nucleic acids. We have evaluated the effect of t...

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Detalles Bibliográficos
Autores principales: Zhang, Lei, Hurek, Thomas, Reinhold-Hurek, Barbara
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1275592/
https://www.ncbi.nlm.nih.gov/pubmed/16257977
http://dx.doi.org/10.1093/nar/gni156