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A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease
A stable heterodimeric protein containing a single correctly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing intein system. As predicted by a model presented earlier, purified SCD protein acts a non-specific nicking endonuclease on normal linear DNA. The...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1277808/ https://www.ncbi.nlm.nih.gov/pubmed/16264086 http://dx.doi.org/10.1093/nar/gki921 |