Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation

BACKGROUND: The objectives of this study were to develop an easy and rapid method for measuring gene expression in a small number of cells by real-time PCR without RNA extraction and purification, and to use this method to determine more precisely IGF-I gene expression in the cumulus cells surroundi...

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Detalles Bibliográficos
Autores principales: Kumamoto, Kanako, Wang, Haifeng, Yamashiro, Hideaki, Terada, Takato
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1291399/
https://www.ncbi.nlm.nih.gov/pubmed/16250920
http://dx.doi.org/10.1186/1477-7827-3-59

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1291399/
https://www.ncbi.nlm.nih.gov/pubmed/16250920
http://dx.doi.org/10.1186/1477-7827-3-59

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