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Easy and rapid method for the determination of gene expression in cumulus cells incubated for oocyte maturation
BACKGROUND: The objectives of this study were to develop an easy and rapid method for measuring gene expression in a small number of cells by real-time PCR without RNA extraction and purification, and to use this method to determine more precisely IGF-I gene expression in the cumulus cells surroundi...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1291399/ https://www.ncbi.nlm.nih.gov/pubmed/16250920 http://dx.doi.org/10.1186/1477-7827-3-59 |