Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability

Quantitative real-time PCR has become the method of choice for measuring mRNA transcription. Recently, fast PCR protocols have been developed as a means to increase assay throughput. Yet it is unclear whether more rapid cycling conditions preserve the original assay performance characteristics. We c...

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Detalles Bibliográficos
Autores principales: Hilscher, Chelsey, Vahrson, Wolfgang, Dittmer, Dirk P.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1297710/
https://www.ncbi.nlm.nih.gov/pubmed/16314296
http://dx.doi.org/10.1093/nar/gni181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1297710/
https://www.ncbi.nlm.nih.gov/pubmed/16314296
http://dx.doi.org/10.1093/nar/gni181

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