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Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
Quantitative real-time PCR has become the method of choice for measuring mRNA transcription. Recently, fast PCR protocols have been developed as a means to increase assay throughput. Yet it is unclear whether more rapid cycling conditions preserve the original assay performance characteristics. We c...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1297710/ https://www.ncbi.nlm.nih.gov/pubmed/16314296 http://dx.doi.org/10.1093/nar/gni181 |