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Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells

BACKGROUND: It is known that aurora B, a chromosomal passenger protein responsible for the proper progression of mitosis and cytokinesis, is overexpressed throughout the cell cycle in cancer cells. Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that...

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Autores principales: Abdullah, Ash-shafie, Foong, Charlene, Murata-Hori, Maki
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298314/
https://www.ncbi.nlm.nih.gov/pubmed/16281968
http://dx.doi.org/10.1186/1475-2867-5-31
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author Abdullah, Ash-shafie
Foong, Charlene
Murata-Hori, Maki
author_facet Abdullah, Ash-shafie
Foong, Charlene
Murata-Hori, Maki
author_sort Abdullah, Ash-shafie
collection PubMed
description BACKGROUND: It is known that aurora B, a chromosomal passenger protein responsible for the proper progression of mitosis and cytokinesis, is overexpressed throughout the cell cycle in cancer cells. Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. However, the detailed dynamics and functions of overexpressed aurora B are poorly understood. RESULTS: We overexpressed GFP fused aurora B kinase in normal rat kidney epithelial cells. Using spinning disk confocal microscopy, we found that overexpressed aurora B-GFP was predominantly localized in the nucleus and along the cortex as a dot-like or short filamentous structure during interphase. Time-lapse imaging revealed that a cytoplasmic fraction of overexpressed aurora B-GFP was incorporated into the nucleus after cell division. Immunofluorescence studies showed that the nuclear fraction of overexpressed aurora B did not induce ectopic phosphorylation of histone H3 after cell division. The cytoplasmic fraction of overexpressed aurora B-GFP was mainly associated with cortical actin filaments but not stress fibers. Myosin II regulatory light chain, one of the possible targets for aurora B, did not colocalize with cortical aurora B-GFP, suggesting that overexpressed aurora B did not promote phosphorylation of myosin II regulatory light chain in interphase cells. CONCLUSION: We conclude that overexpressed aurora B has a specific localization pattern in interphase cells. Based on our findings, we propose that overexpressed aurora B targets the nuclear and cortical proteins during interphase, which may contribute to cancer development and tumor metastasis.
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spelling pubmed-12983142006-11-24 Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells Abdullah, Ash-shafie Foong, Charlene Murata-Hori, Maki Cancer Cell Int Primary Research BACKGROUND: It is known that aurora B, a chromosomal passenger protein responsible for the proper progression of mitosis and cytokinesis, is overexpressed throughout the cell cycle in cancer cells. Overexpression of aurora B produced multinuclearity and induced aggressive metastasis, suggesting that overexpressed aurora B has multiple functions in cancer development. However, the detailed dynamics and functions of overexpressed aurora B are poorly understood. RESULTS: We overexpressed GFP fused aurora B kinase in normal rat kidney epithelial cells. Using spinning disk confocal microscopy, we found that overexpressed aurora B-GFP was predominantly localized in the nucleus and along the cortex as a dot-like or short filamentous structure during interphase. Time-lapse imaging revealed that a cytoplasmic fraction of overexpressed aurora B-GFP was incorporated into the nucleus after cell division. Immunofluorescence studies showed that the nuclear fraction of overexpressed aurora B did not induce ectopic phosphorylation of histone H3 after cell division. The cytoplasmic fraction of overexpressed aurora B-GFP was mainly associated with cortical actin filaments but not stress fibers. Myosin II regulatory light chain, one of the possible targets for aurora B, did not colocalize with cortical aurora B-GFP, suggesting that overexpressed aurora B did not promote phosphorylation of myosin II regulatory light chain in interphase cells. CONCLUSION: We conclude that overexpressed aurora B has a specific localization pattern in interphase cells. Based on our findings, we propose that overexpressed aurora B targets the nuclear and cortical proteins during interphase, which may contribute to cancer development and tumor metastasis. BioMed Central 2005-11-09 /pmc/articles/PMC1298314/ /pubmed/16281968 http://dx.doi.org/10.1186/1475-2867-5-31 Text en Copyright © 2005 Abdullah et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primary Research
Abdullah, Ash-shafie
Foong, Charlene
Murata-Hori, Maki
Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title_full Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title_fullStr Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title_full_unstemmed Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title_short Specific distribution of overexpressed aurora B kinase in interphase normal epithelial cells
title_sort specific distribution of overexpressed aurora b kinase in interphase normal epithelial cells
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298314/
https://www.ncbi.nlm.nih.gov/pubmed/16281968
http://dx.doi.org/10.1186/1475-2867-5-31
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