Cargando…

Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)

cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5′- and 3′-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and protein...

Descripción completa

Detalles Bibliográficos
Autores principales: Hoskins, Roger A., Stapleton, Mark, George, Reed A., Yu, Charles, Wan, Kenneth H., Carlson, Joseph W., Celniker, Susan E.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1301602/
https://www.ncbi.nlm.nih.gov/pubmed/16326860
http://dx.doi.org/10.1093/nar/gni184