Cargando…
Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)
cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5′- and 3′-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and protein...
Autores principales: | Hoskins, Roger A., Stapleton, Mark, George, Reed A., Yu, Charles, Wan, Kenneth H., Carlson, Joseph W., Celniker, Susan E. |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1301602/ https://www.ncbi.nlm.nih.gov/pubmed/16326860 http://dx.doi.org/10.1093/nar/gni184 |
Ejemplares similares
-
Screening of retroviral cDNA libraries for factors involved in protein phosphorylation in signaling cascades
por: Stitz, J., et al.
Publicado: (2005) -
Non-biased and efficient global amplification of a single-cell cDNA library
por: Huang, Huan, et al.
Publicado: (2014) -
An efficient and sensitive method for preparing cDNA libraries from scarce biological samples
por: Sterling, Catherine H., et al.
Publicado: (2015) -
Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification
por: Tougan, Takahiro, et al.
Publicado: (2008) -
An on-bead tailing/ligation approach for sequencing resin-bound RNA libraries
por: Wiesmayr, Anna, et al.
Publicado: (2012)