Touchdown General Primer (GP5+/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus

BACKGROUND: The GP5+/GP6+ PCR assay is a well-established HPV detection technique. This study has examined the effects of incorporating 'hot start' and 'touchdown' steps into the protocol. In addition, dTTP was substituted with dUTP to permit contamination control measures agains...

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Detalles Bibliográficos
Autores principales: Evans, Mark F, Adamson, Christine SC, Simmons-Arnold, Linda, Cooper, Kumarasen
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1314887/
https://www.ncbi.nlm.nih.gov/pubmed/16288661
http://dx.doi.org/10.1186/1472-6890-5-10

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1314887/
https://www.ncbi.nlm.nih.gov/pubmed/16288661
http://dx.doi.org/10.1186/1472-6890-5-10

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