Cargando…
Development of antibodies to human embryonic stem cell antigens
BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1315352/ https://www.ncbi.nlm.nih.gov/pubmed/16316465 http://dx.doi.org/10.1186/1471-213X-5-26 |
_version_ | 1782126385717837824 |
---|---|
author | Cai, Jingli Olson, Judith M Rao, Mahendra S Stanley, Marisa Taylor, Eva Ni, Hsiao-Tzu |
author_facet | Cai, Jingli Olson, Judith M Rao, Mahendra S Stanley, Marisa Taylor, Eva Ni, Hsiao-Tzu |
author_sort | Cai, Jingli |
collection | PubMed |
description | BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage. RESULTS: In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage. CONCLUSION: These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation. |
format | Text |
id | pubmed-1315352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-13153522005-12-16 Development of antibodies to human embryonic stem cell antigens Cai, Jingli Olson, Judith M Rao, Mahendra S Stanley, Marisa Taylor, Eva Ni, Hsiao-Tzu BMC Dev Biol Methodology Article BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage. RESULTS: In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage. CONCLUSION: These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation. BioMed Central 2005-11-29 /pmc/articles/PMC1315352/ /pubmed/16316465 http://dx.doi.org/10.1186/1471-213X-5-26 Text en Copyright © 2005 Cai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Cai, Jingli Olson, Judith M Rao, Mahendra S Stanley, Marisa Taylor, Eva Ni, Hsiao-Tzu Development of antibodies to human embryonic stem cell antigens |
title | Development of antibodies to human embryonic stem cell antigens |
title_full | Development of antibodies to human embryonic stem cell antigens |
title_fullStr | Development of antibodies to human embryonic stem cell antigens |
title_full_unstemmed | Development of antibodies to human embryonic stem cell antigens |
title_short | Development of antibodies to human embryonic stem cell antigens |
title_sort | development of antibodies to human embryonic stem cell antigens |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1315352/ https://www.ncbi.nlm.nih.gov/pubmed/16316465 http://dx.doi.org/10.1186/1471-213X-5-26 |
work_keys_str_mv | AT caijingli developmentofantibodiestohumanembryonicstemcellantigens AT olsonjudithm developmentofantibodiestohumanembryonicstemcellantigens AT raomahendras developmentofantibodiestohumanembryonicstemcellantigens AT stanleymarisa developmentofantibodiestohumanembryonicstemcellantigens AT tayloreva developmentofantibodiestohumanembryonicstemcellantigens AT nihsiaotzu developmentofantibodiestohumanembryonicstemcellantigens |