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Development of antibodies to human embryonic stem cell antigens

BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/...

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Detalles Bibliográficos
Autores principales: Cai, Jingli, Olson, Judith M, Rao, Mahendra S, Stanley, Marisa, Taylor, Eva, Ni, Hsiao-Tzu
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1315352/
https://www.ncbi.nlm.nih.gov/pubmed/16316465
http://dx.doi.org/10.1186/1471-213X-5-26
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author Cai, Jingli
Olson, Judith M
Rao, Mahendra S
Stanley, Marisa
Taylor, Eva
Ni, Hsiao-Tzu
author_facet Cai, Jingli
Olson, Judith M
Rao, Mahendra S
Stanley, Marisa
Taylor, Eva
Ni, Hsiao-Tzu
author_sort Cai, Jingli
collection PubMed
description BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage. RESULTS: In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage. CONCLUSION: These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation.
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spelling pubmed-13153522005-12-16 Development of antibodies to human embryonic stem cell antigens Cai, Jingli Olson, Judith M Rao, Mahendra S Stanley, Marisa Taylor, Eva Ni, Hsiao-Tzu BMC Dev Biol Methodology Article BACKGROUND: Using antibodies to specific protein antigens is the method of choice to assign and identify cell lineage through simultaneous analysis of surface molecules and intracellular markers. Embryonic stem cell research can be benefited from using antibodies specific to transcriptional factors/markers that contribute to the "stemness" phenotype or critical for cell lineage. RESULTS: In this report, we have developed and validated antibodies (either monoclonal or polyclonal) specific to human embryonic stem cell antigens and early differentiation transcriptional factors/markers that are critical for cell differentiation into definite lineage. CONCLUSION: These antibodies enable stem cell biologists to conveniently identify stem cell characteristics and to quantitatively assess differentiation. BioMed Central 2005-11-29 /pmc/articles/PMC1315352/ /pubmed/16316465 http://dx.doi.org/10.1186/1471-213X-5-26 Text en Copyright © 2005 Cai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Cai, Jingli
Olson, Judith M
Rao, Mahendra S
Stanley, Marisa
Taylor, Eva
Ni, Hsiao-Tzu
Development of antibodies to human embryonic stem cell antigens
title Development of antibodies to human embryonic stem cell antigens
title_full Development of antibodies to human embryonic stem cell antigens
title_fullStr Development of antibodies to human embryonic stem cell antigens
title_full_unstemmed Development of antibodies to human embryonic stem cell antigens
title_short Development of antibodies to human embryonic stem cell antigens
title_sort development of antibodies to human embryonic stem cell antigens
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1315352/
https://www.ncbi.nlm.nih.gov/pubmed/16316465
http://dx.doi.org/10.1186/1471-213X-5-26
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