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Standardisation of data from real-time quantitative PCR methods – evaluation of outliers and comparison of calibration curves

BACKGROUND: As real-time quantitative PCR (RT-QPCR) is increasingly being relied upon for the enforcement of legislation and regulations dependent upon the trace detection of DNA, focus has increased on the quality issues related to the technique. Recent work has focused on the identification of fac...

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Detalles Bibliográficos
Autores principales: Burns, Malcolm J, Nixon, Gavin J, Foy, Carole A, Harris, Neil
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326201/
https://www.ncbi.nlm.nih.gov/pubmed/16336641
http://dx.doi.org/10.1186/1472-6750-5-31