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Standardisation of data from real-time quantitative PCR methods – evaluation of outliers and comparison of calibration curves
BACKGROUND: As real-time quantitative PCR (RT-QPCR) is increasingly being relied upon for the enforcement of legislation and regulations dependent upon the trace detection of DNA, focus has increased on the quality issues related to the technique. Recent work has focused on the identification of fac...
Autores principales: | Burns, Malcolm J, Nixon, Gavin J, Foy, Carole A, Harris, Neil |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326201/ https://www.ncbi.nlm.nih.gov/pubmed/16336641 http://dx.doi.org/10.1186/1472-6750-5-31 |
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