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Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors

BACKGROUND: The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the target protein with a suitable partner. Despite several reports on the success...

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Detalles Bibliográficos
Autores principales: Dümmler, Annett, Lawrence, Ann-Marie, de Marco, Ario
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326211/
https://www.ncbi.nlm.nih.gov/pubmed/16351710
http://dx.doi.org/10.1186/1475-2859-4-34