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Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors
BACKGROUND: The solubility of recombinant proteins expressed in bacteria is often disappointingly low. Several strategies have been developed to improve the yield and one of the most common strategies is the fusion of the target protein with a suitable partner. Despite several reports on the success...
Autores principales: | Dümmler, Annett, Lawrence, Ann-Marie, de Marco, Ario |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1326211/ https://www.ncbi.nlm.nih.gov/pubmed/16351710 http://dx.doi.org/10.1186/1475-2859-4-34 |
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