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Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants

BACKGROUND: We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vec...

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Detalles Bibliográficos
Autores principales: Hellens, Roger P, Allan, Andrew C, Friel, Ellen N, Bolitho, Karen, Grafton, Karryn, Templeton, Matthew D, Karunairetnam, Sakuntala, Gleave, Andrew P, Laing, William A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1334188/
https://www.ncbi.nlm.nih.gov/pubmed/16359558
http://dx.doi.org/10.1186/1746-4811-1-13