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Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line

During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation...

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Detalles Bibliográficos
Autores principales:	Kanayama, Naoki, Todo, Kagefumi, Takahashi, Satoko, Magari, Masaki, Ohmori, Hitoshi
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1342040/ https://www.ncbi.nlm.nih.gov/pubmed/16421270 http://dx.doi.org/10.1093/nar/gnj013

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