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A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast

The technologies for chromosome modification developed to date are not satisfactorily universal, owing to the typical requirements for special enzymes and sequences. In the present report, we propose a new approach for chromosome modification in Schizosaccharomyces pombe that does not involve any sp...

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Detalles Bibliográficos
Autores principales: Hirashima, Kyotaro, Iwaki, Tomoko, Takegawa, Kaoru, Giga-Hama, Yuko, Tohda, Hideki
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1351375/
https://www.ncbi.nlm.nih.gov/pubmed/16434698
http://dx.doi.org/10.1093/nar/gnj011