Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants

Restriction endonucleases (REases) with 8-base specificity are rare specimens in nature. NotI from Nocardia otitidis-caviarum (recognition sequence 5′-GCGGCCGC-3′) has been cloned, thus allowing for mutagenesis and screening for enzymes with altered 8-base recognition and cleavage activity. Variants...

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Detalles Bibliográficos
Autores principales: Samuelson, James C., Morgan, Richard D., Benner, Jack S., Claus, Toby E., Packard, Stephanie L., Xu, Shuang-yong
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360745/
https://www.ncbi.nlm.nih.gov/pubmed/16456032
http://dx.doi.org/10.1093/nar/gkj483

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360745/
https://www.ncbi.nlm.nih.gov/pubmed/16456032
http://dx.doi.org/10.1093/nar/gkj483

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