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Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi
BACKGROUND: Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene s...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363358/ https://www.ncbi.nlm.nih.gov/pubmed/16381610 http://dx.doi.org/10.1186/1471-213X-5-28 |
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author | Soares, Miguel L Haraguchi, Seiki Torres-Padilla, Maria-Elena Kalmar, Tibor Carpenter, Lee Bell, Graham Morrison, Alastair Ring, Christopher JA Clarke, Neil J Glover, David M Zernicka-Goetz, Magdalena |
author_facet | Soares, Miguel L Haraguchi, Seiki Torres-Padilla, Maria-Elena Kalmar, Tibor Carpenter, Lee Bell, Graham Morrison, Alastair Ring, Christopher JA Clarke, Neil J Glover, David M Zernicka-Goetz, Magdalena |
author_sort | Soares, Miguel L |
collection | PubMed |
description | BACKGROUND: Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene silencing in late cleavage-stage mouse embryos that permits examination of phenotypes at post-implantation stages. RESULTS: We show that introduction of Bmp4 dsRNA into intact blastocysts by electroporation recapitulates the genetic Bmp4 null phenotype at gastrulation. It also reveals a novel role for Bmp4 in the regulation the anterior visceral endoderm specific gene expression and its positioning. We also show that RNAi can be used to simultaneously target several genes. When applied to the three murine isoforms of Dishevelled, it leads to earlier defects than previously observed in double knock-outs. These include severe delays in post-implantation development and defects in the anterior midline and neural folds at headfold stages. CONCLUSION: Our results indicate that the BMP4 signalling pathway contributes to the development of the anterior visceral endoderm, and reveal an early functional redundancy between the products of the murine Dishevelled genes. The proposed approach constitutes a powerful tool to screen the functions of genes that govern the development of the mouse embryo. |
format | Text |
id | pubmed-1363358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-13633582006-02-10 Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi Soares, Miguel L Haraguchi, Seiki Torres-Padilla, Maria-Elena Kalmar, Tibor Carpenter, Lee Bell, Graham Morrison, Alastair Ring, Christopher JA Clarke, Neil J Glover, David M Zernicka-Goetz, Magdalena BMC Dev Biol Research Article BACKGROUND: Studies of gene function in the mouse have relied mainly on gene targeting via homologous recombination. However, this approach is difficult to apply in specific windows of time, and to simultaneously knock-down multiple genes. Here we report an efficient method for dsRNA-mediated gene silencing in late cleavage-stage mouse embryos that permits examination of phenotypes at post-implantation stages. RESULTS: We show that introduction of Bmp4 dsRNA into intact blastocysts by electroporation recapitulates the genetic Bmp4 null phenotype at gastrulation. It also reveals a novel role for Bmp4 in the regulation the anterior visceral endoderm specific gene expression and its positioning. We also show that RNAi can be used to simultaneously target several genes. When applied to the three murine isoforms of Dishevelled, it leads to earlier defects than previously observed in double knock-outs. These include severe delays in post-implantation development and defects in the anterior midline and neural folds at headfold stages. CONCLUSION: Our results indicate that the BMP4 signalling pathway contributes to the development of the anterior visceral endoderm, and reveal an early functional redundancy between the products of the murine Dishevelled genes. The proposed approach constitutes a powerful tool to screen the functions of genes that govern the development of the mouse embryo. BioMed Central 2005-12-28 /pmc/articles/PMC1363358/ /pubmed/16381610 http://dx.doi.org/10.1186/1471-213X-5-28 Text en Copyright © 2005 Soares et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Soares, Miguel L Haraguchi, Seiki Torres-Padilla, Maria-Elena Kalmar, Tibor Carpenter, Lee Bell, Graham Morrison, Alastair Ring, Christopher JA Clarke, Neil J Glover, David M Zernicka-Goetz, Magdalena Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title | Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title_full | Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title_fullStr | Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title_full_unstemmed | Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title_short | Functional studies of signaling pathways in peri-implantation development of the mouse embryo by RNAi |
title_sort | functional studies of signaling pathways in peri-implantation development of the mouse embryo by rnai |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363358/ https://www.ncbi.nlm.nih.gov/pubmed/16381610 http://dx.doi.org/10.1186/1471-213X-5-28 |
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