Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage φC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the co...

Descripción completa

Detalles Bibliográficos
Autores principales: Khodakaramian, Gholam, Lissenden, Sarah, Gust, Bertolt, Moir, Laura, Hoskisson, Paul A., Chater, Keith F., Smith, Margaret C. M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781/
https://www.ncbi.nlm.nih.gov/pubmed/16473843
http://dx.doi.org/10.1093/nar/gnj019

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363781/
https://www.ncbi.nlm.nih.gov/pubmed/16473843
http://dx.doi.org/10.1093/nar/gnj019

Ejemplares similares