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BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5′-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized the derivatized surfaces for the chemical at...

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Detalles Bibliográficos
Autores principales: Fedurco, Milan, Romieu, Anthony, Williams, Scott, Lawrence, Isabelle, Turcatti, Gerardo
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363783/
https://www.ncbi.nlm.nih.gov/pubmed/16473845
http://dx.doi.org/10.1093/nar/gnj023