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BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies
The tricarboxylate reagent benzene-1,3,5-triacetic acid (BTA) was used to attach 5′-aminated DNA primers and templates on an aminosilanized glass surface for subsequent generation of DNA colonies by in situ solid-phase amplification. We have characterized the derivatized surfaces for the chemical at...
Autores principales: | Fedurco, Milan, Romieu, Anthony, Williams, Scott, Lawrence, Isabelle, Turcatti, Gerardo |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1363783/ https://www.ncbi.nlm.nih.gov/pubmed/16473845 http://dx.doi.org/10.1093/nar/gnj023 |
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