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Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs
For many years, Taq polymerase has served as the stalwart enzyme in the PCR amplification of DNA. However, a major limitation of Taq is its inability to amplify damaged DNA, thereby restricting its usefulness in forensic applications. In contrast, Y-family DNA polymerases, such as Dpo4 from Sulfolob...
Autores principales: | McDonald, John P., Hall, Ashley, Gasparutto, Didier, Cadet, Jean, Ballantyne, Jack, Woodgate, Roger |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1373694/ https://www.ncbi.nlm.nih.gov/pubmed/16488882 http://dx.doi.org/10.1093/nar/gkj512 |
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