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Estimating the number of integrations in transformed plants by quantitative real-time PCR

BACKGROUND: When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this can deeply influence the level of transgene expression and the ease of stabili...

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Detalles Bibliográficos
Autores principales: Mason, Giovanna, Provero, Paolo, Vaira, Anna Maria, Accotto, Gian Paolo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC137580/
https://www.ncbi.nlm.nih.gov/pubmed/12398792
http://dx.doi.org/10.1186/1472-6750-2-20