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Estimating the number of integrations in transformed plants by quantitative real-time PCR
BACKGROUND: When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this can deeply influence the level of transgene expression and the ease of stabili...
Autores principales: | Mason, Giovanna, Provero, Paolo, Vaira, Anna Maria, Accotto, Gian Paolo |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC137580/ https://www.ncbi.nlm.nih.gov/pubmed/12398792 http://dx.doi.org/10.1186/1472-6750-2-20 |
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