Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii

The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility...

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Autores principales: Hu, Ke, Johnson, Jeff, Florens, Laurence, Fraunholz, Martin, Suravajjala, Sapna, DiLullo, Camille, Yates, John, Roos, David S, Murray, John M
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383488/
https://www.ncbi.nlm.nih.gov/pubmed/16518471
http://dx.doi.org/10.1371/journal.ppat.0020013
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author Hu, Ke
Johnson, Jeff
Florens, Laurence
Fraunholz, Martin
Suravajjala, Sapna
DiLullo, Camille
Yates, John
Roos, David S
Murray, John M
author_facet Hu, Ke
Johnson, Jeff
Florens, Laurence
Fraunholz, Martin
Suravajjala, Sapna
DiLullo, Camille
Yates, John
Roos, David S
Murray, John M
author_sort Hu, Ke
collection PubMed
description The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified ~200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication.
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spelling pubmed-13834882007-09-22 Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii Hu, Ke Johnson, Jeff Florens, Laurence Fraunholz, Martin Suravajjala, Sapna DiLullo, Camille Yates, John Roos, David S Murray, John M PLoS Pathog Research Article The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified ~200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication. Public Library of Science 2006-02 2006-02-24 /pmc/articles/PMC1383488/ /pubmed/16518471 http://dx.doi.org/10.1371/journal.ppat.0020013 Text en © 2006 Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Ke
Johnson, Jeff
Florens, Laurence
Fraunholz, Martin
Suravajjala, Sapna
DiLullo, Camille
Yates, John
Roos, David S
Murray, John M
Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title_full Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title_fullStr Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title_full_unstemmed Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title_short Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
title_sort cytoskeletal components of an invasion machine—the apical complex of toxoplasma gondii
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383488/
https://www.ncbi.nlm.nih.gov/pubmed/16518471
http://dx.doi.org/10.1371/journal.ppat.0020013
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