Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383488/ https://www.ncbi.nlm.nih.gov/pubmed/16518471 http://dx.doi.org/10.1371/journal.ppat.0020013 |
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author | Hu, Ke Johnson, Jeff Florens, Laurence Fraunholz, Martin Suravajjala, Sapna DiLullo, Camille Yates, John Roos, David S Murray, John M |
author_facet | Hu, Ke Johnson, Jeff Florens, Laurence Fraunholz, Martin Suravajjala, Sapna DiLullo, Camille Yates, John Roos, David S Murray, John M |
author_sort | Hu, Ke |
collection | PubMed |
description | The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified ~200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication. |
format | Text |
id | pubmed-1383488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-13834882007-09-22 Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii Hu, Ke Johnson, Jeff Florens, Laurence Fraunholz, Martin Suravajjala, Sapna DiLullo, Camille Yates, John Roos, David S Murray, John M PLoS Pathog Research Article The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified ~200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication. Public Library of Science 2006-02 2006-02-24 /pmc/articles/PMC1383488/ /pubmed/16518471 http://dx.doi.org/10.1371/journal.ppat.0020013 Text en © 2006 Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Ke Johnson, Jeff Florens, Laurence Fraunholz, Martin Suravajjala, Sapna DiLullo, Camille Yates, John Roos, David S Murray, John M Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii |
title | Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
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title_full | Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
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title_fullStr | Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
|
title_full_unstemmed | Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
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title_short | Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii
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title_sort | cytoskeletal components of an invasion machine—the apical complex of toxoplasma gondii |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1383488/ https://www.ncbi.nlm.nih.gov/pubmed/16518471 http://dx.doi.org/10.1371/journal.ppat.0020013 |
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