A Method for Assaying Deubiquitinating Enzymes

A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused αNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a...

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Detalles Bibliográficos
Autores principales: Lee, Jae Il, Woo, Seung Kyoon, Kim, Keun Il, Park, Kyung Chan, Baek, Sung Hee, Yoo, Yung Joon, Chung, Chin Ha
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 1998
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140113/
https://www.ncbi.nlm.nih.gov/pubmed/12734599
http://dx.doi.org/10.1251/bpo11

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140113/
https://www.ncbi.nlm.nih.gov/pubmed/12734599
http://dx.doi.org/10.1251/bpo11

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