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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
1998
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140120/ https://www.ncbi.nlm.nih.gov/pubmed/12734592 http://dx.doi.org/10.1251/bpo4 |