Cargando…
Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic...
Autores principales: | Becerra, M, Cerdãn, E, Gonzãlez Siso, MI |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
1998
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140120/ https://www.ncbi.nlm.nih.gov/pubmed/12734592 http://dx.doi.org/10.1251/bpo4 |
Ejemplares similares
-
Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
por: Rodríguez, Ángel Pereira, et al.
Publicado: (2006) -
Rational mutagenesis by engineering disulphide bonds improves Kluyveromyces lactis beta-galactosidase for high-temperature industrial applications
por: Rico-Díaz, Agustín, et al.
Publicado: (2017) -
Sugar metabolism, redox balance and oxidative stress response in the respiratory yeast Kluyveromyces lactis
por: González-Siso, M Isabel, et al.
Publicado: (2009) -
Kluyveromyces lactis: A Suitable Yeast Model to Study Cellular Defense Mechanisms against Hypoxia-Induced Oxidative Stress
por: González Siso, M. Isabel, et al.
Publicado: (2012) -
Application of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside
por: Wojciechowska, Aleksandra, et al.
Publicado: (2017)