RecET driven chromosomal gene targeting to generate a RecA deficient Escherichia coli strain for Cre mediated production of minicircle DNA

BACKGROUND: Minicircle DNA is the non-replicating product of intramolecular site-specific recombination within a bacterial minicircle producer plasmid. Minicircle DNA can be engineered to contain predominantly human sequences which have a low content of CpG dinucleotides and thus reduced immunotoxic...

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Detalles Bibliográficos
Autores principales: Tolmachov, Oleg, Palaszewski, Iwona, Bigger, Brian, Coutelle, Charles
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421399/
https://www.ncbi.nlm.nih.gov/pubmed/16529656
http://dx.doi.org/10.1186/1472-6750-6-17

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421399/
https://www.ncbi.nlm.nih.gov/pubmed/16529656
http://dx.doi.org/10.1186/1472-6750-6-17

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