Experimental and computational investigations of Ser10 and Lys13 in the binding and cleavage of DNA substrates by Escherichia coli DNA topoisomerase I

Ser10 and Lys13 found near the active site tyrosine of Escherichia coli DNA topoisomerase I are conserved among the type IA topoisomerases. Site-directed mutagenesis of these two residues to Ala reduced the relaxation and DNA cleavage activity, with a more severe effect from the Lys13 mutation. Chan...

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Detalles Bibliográficos
Autores principales: Strahs, Daniel, Zhu, Chang-Xi, Cheng, Bokun, Chen, Jason, Tse-Dinh, Yuk-Ching
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421505/
https://www.ncbi.nlm.nih.gov/pubmed/16582104
http://dx.doi.org/10.1093/nar/gkl109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1421505/
https://www.ncbi.nlm.nih.gov/pubmed/16582104
http://dx.doi.org/10.1093/nar/gkl109

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