Cargando…

Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155

Vector-based RNA interference (RNAi) has emerged as a valuable tool for analysis of gene function. We have developed new RNA polymerase II expression vectors for RNAi, designated SIBR vectors, based upon the non-coding RNA BIC. BIC contains the miR-155 microRNA (miRNA) precursor, and we find that ex...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chung, Kwan-Ho, Hart, Christopher C., Al-Bassam, Sarmad, Avery, Adam, Taylor, Jennifer, Patel, Paresh D., Vojtek, Anne B., Turner, David L.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1435982/ https://www.ncbi.nlm.nih.gov/pubmed/16614444 http://dx.doi.org/10.1093/nar/gkl143

Ejemplares similares

RNA polymerase III can drive polycistronic expression of functional interfering RNAs designed to resemble microRNAs
por: Snyder, Lindsey L., et al.
Publicado: (2009)

Control of small inhibitory RNA levels and RNA interference by doxycycline induced activation of a minimal RNA polymerase III promoter
por: Amar, Lahouari, et al.
Publicado: (2006)

Development of a species-specific RNA polymerase I-based shRNA expression vector
por: Verca, M. S. Brenz, et al.
Publicado: (2007)

Improvement of reporter activity by IRES-mediated polycistronic reporter system
por: Bouabe, Hicham, et al.
Publicado: (2008)

Isoform-level microRNA-155 target prediction using RNA-seq
por: Deng, Nan, et al.
Publicado: (2011)

CellBIC: bimodality-based top-down clustering of single-cell RNA sequencing data reveals hierarchical structure of the cell type
por: Kim, Junil, et al.
Publicado: (2018)

Synthesis of 2′-Fluoro RNA by Syn5 RNA polymerase
por: Zhu, Bin, et al.
Publicado: (2015)

Reconstitution of human RNA interference in budding yeast
por: Suk, Kyoungho, et al.
Publicado: (2011)

SnoVectors for nuclear expression of RNA
por: Yin, Qing-Fei, et al.
Publicado: (2015)

An inducible RNA interference system for the functional dissection of mouse embryogenesis
por: Vidigal, Joana A., et al.
Publicado: (2010)

(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
por: Neguembor, Maria V, et al.
Publicado: (2018)

Efficient inhibition of RNA self-primed extension by addition of competing 3′-capture DNA-improved RNA synthesis by T7 RNA polymerase
por: Gholamalipour, Yasaman, et al.
Publicado: (2019)

RNA interference by mixtures of siRNAs prepared using custom oligonucleotide arrays
por: Oleinikov, Andrew V., et al.
Publicado: (2005)

An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi
por: Zhou, Hongxia, et al.
Publicado: (2005)

A bi-functional siRNA construct induces RNA interference and also primes PCR amplification for its own quantification
por: Jiang, Ming, et al.
Publicado: (2005)

Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus
por: Anesti, Anna-Maria, et al.
Publicado: (2008)

p53-dependent antiviral RNA-interference facilitates tumor-selective viral replication
por: Gürlevik, Engin, et al.
Publicado: (2009)

Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibition
por: Blazquez, Lorea, et al.
Publicado: (2012)

Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression
por: Abad, X., et al.
Publicado: (2010)

Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference
por: Hitz, Christiane, et al.
Publicado: (2007)

Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference
por: Coumoul, Xavier, et al.
Publicado: (2005)

A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for microRNA interference
por: Lu, Yanjie, et al.
Publicado: (2009)

Inducible microRNA expression by an all-in-one episomal vector system
por: Epanchintsev, Alexey, et al.
Publicado: (2006)

Boiler: lossy compression of RNA-seq alignments using coverage vectors
por: Pritt, Jacob, et al.
Publicado: (2016)

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme
por: Asahara, Haruichi, et al.
Publicado: (2010)

Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells
por: Haraguchi, Takeshi, et al.
Publicado: (2009)

A genetic screen for components of the mammalian RNA interference pathway in Bloom-deficient mouse embryonic stem cells
por: Trombly, Melanie I., et al.
Publicado: (2009)

Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification
por: van Bakel, Harm, et al.
Publicado: (2008)

Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay
por: Cheng, Bo, et al.
Publicado: (2008)

POLYPHEMUS: R package for comparative analysis of RNA polymerase II ChIP-seq profiles by non-linear normalization
por: Mendoza-Parra, Marco A., et al.
Publicado: (2012)

Efficient production of superior dumbbell-shaped DNA minimal vectors for small hairpin RNA expression
por: Yu, Han, et al.
Publicado: (2015)

Single small-interfering RNA expression vector for silencing multiple transforming growth factor-β pathway components
por: Jazag, Amarsanaa, et al.
Publicado: (2005)

Improved knockdown from artificial microRNAs in an enhanced miR-155 backbone: a designer's guide to potent multi-target RNAi
por: Fowler, Daniel K., et al.
Publicado: (2016)

Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry
por: Winz, Marie-Luise, et al.
Publicado: (2012)

In cell mutational interference mapping experiment (in cell MIME) identifies the 5′ polyadenylation signal as a dual regulator of HIV-1 genomic RNA production and packaging
por: Smyth, Redmond P, et al.
Publicado: (2018)

Specific ligation to double-stranded RNA for analysis of cellular RNA::RNA interactions
por: Faridani, Omid R., et al.
Publicado: (2008)

‘RNA walk’ a novel approach to study RNA–RNA interactions between a small RNA and its target
por: Lustig, Yaniv, et al.
Publicado: (2010)

A polymerase engineered for bisulfite sequencing
por: Millar, Doug, et al.
Publicado: (2015)

Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot
por: Pall, Gurman Singh, et al.
Publicado: (2007)

Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
por: Jozwiakowski, Stanislaw K., et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_kdqarcug30ako0ldc48c9sno1l