Cargando…

"Per cell" normalization method for mRNA measurement by quantitative PCR and microarrays

BACKGROUND: Transcriptome data from quantitative PCR (Q-PCR) and DNA microarrays are typically obtained from a fixed amount of RNA collected per sample. Therefore, variations in tissue cellularity and RNA yield across samples in an experimental series compromise accurate determination of the absolut...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanno, Jun, Aisaki, Ken-ichi, Igarashi, Katsuhide, Nakatsu, Noriyuki, Ono, Atsushi, Kodama, Yukio, Nagao, Taku
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448209/
https://www.ncbi.nlm.nih.gov/pubmed/16571132
http://dx.doi.org/10.1186/1471-2164-7-64